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STAFF INFORMATION

DR JOHN LEWIS Ph.D.
Section Head, Steroid Chemistry
Phone : 03 364 0300 Ex 80877
Fax : 03 364 0889
EMail : john.lewis@cdhb.govt.nz

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The Steroid and Immunobiochemistry Laboratory at Canterbury Health Laboratories provides a comprehensive analytical service for steroid hormones and related proteins. In addition it develops monoclonal antibodies to steroid hormones and steroid related proteins for use in ELISA based assays.

Publications

  1. LEWIS JG. ,Shand BI. ,Frampton CM. ,Elder PA ,Scott RS.
    ",Plasma retinol-binding protein is not a marker of insulin resistance in overweight subjects: a three year longitudinal study.
    Clin Biochem 2008;Sep;41(13):1034-8.
  2. LEWIS JG. ,Shand BI. ,Elder PA ,Scott RS.
    ",Plasma retinol-binding protein is unlikely to be a useful marker of insulin resistance.
    Diabetes Res Clin Pract 2008;Apr;80(1):e13-5
  3. LEWIS JG. ,Elder PA
    ",Serum 25-OH vitamin D2 and D3 are stable under exaggerated conditions.
    Clin Chem 2008;54(11):1931-2
  4. Ho JT. ,LEWIS JG. ,OLoughlin P. ,Bagley CJ. ,Romero R. ,Dekker GA. ,Torpy DJ.
    ",Reduced maternal corticosteroid-binding globulin and cortisol levels in pre-eclampsia and gamete recipient pregnancies.
    Clin Endocrinol (Oxf). 2007;66(6):869-77
  5. Ho JT. ,Keogh JB. ,Bornstein SR. ,Ehrhart-Bornstein M. ,LEWIS JG. ,Clifton PM. ,Torpy DJ.
    ",Moderate weight loss reduces renin and aldosterone but does not influence basal or stimulated pituitary-adrenal axis function.
    Horm Metab Res 2007;39(9):694-9
  6. Levine A. ,Zagoory-Sharon O. ,Feldman R. ,LEWIS JG. ,Weller A.
    ",Measuring cortisol in human psychobiological studies.
    Physiol Behav. 2007;90(1):43-53
  7. LEWIS JG. ,Shand BI. ,Frampton CM. ,Elder PA
    ",An ELISA for plasma retinol-binding protein using monoclonal and polyclonal antibodies: plasma variation in normal and insulin resistant subjects.
    Clin Biochem. 2007;40(11):828-34
  8. LEWIS JG.
    "Steroid analysis in saliva: an overview."
    Clin Biochem Rev. 2006;27:139-146
  9. LEWIS JG. ,Mopert B. ,Shand BI. ,Doogue MP. ,Soule SG. ,Frampton CM. ,Elder PA
    ",Plasma variation of corticosteroid-binding globulin and sex hormone-binding globulin.
    Horm Metab Res. 2006;38(4):241-5
  10. Sivukhina EV. ,Jirikowski GF. ,Bernstein HG. ,LEWIS JG. ,Herbert Z.
    ",Expression of corticosteroid-binding protein in the human hypothalamus, co-localization with oxytocin and vasopressin.
    Horm Metab Res. 2006;38(4):253-9
  11. Davidson JS. ,Bolland MlJ. ,Croxson MS. ,Chiu W. ,LEWIS JG.
    ",A case of low cortisol-binding globulin: use of plasma free cortisol in interpretation of hypothalamic-pituitary-adrenal axis tests.
    Ann Clin Biochem. 2006;43(Pt 3):237-9
  12. Mihrshahi R. ,LEWIS JG. ,Ali SO.
    ",Hormonal effects on the secretion and glycoform profile of corticosteroid-binding globulin.
    J Steroid Biochem Mol Biol. 2006;101(4-5):275-85
  13. Ho JT. ,Al-Musalhi H. ,Chapman MJ. ,Quach T. ,Thomas PD. ,Bagley CJ. ,LEWIS JG.
    ",Septic shock and sepsis: a comparison of total and free plasma cortisol levels.
    J Clin Endocrinol Metab. 2006;91(1):105-14
  14. Selva DM. ,Bassas L. ,Munell F. ,Mata A. ,Tekpetey F. ,LEWIS JG. ,Hammon GL.
    ",Human sperm sex hormone-binding globulin isoform: characterization and measurement by time-resolved fluorescence immunoassay.
    J Clin Endocrinol Metab. 2005;90(11):6275-82
  15. LEWIS JG. ,Nakajin S. ,OhnoS. ,Warnock A. ,Florkowski CM ,Elder PA
    ",Circulating levels of isoflavones and markers of 5alpha-reductase activity are higher in Japanese compared with New Zealand males: What is the role of circulating steroids in prostate disease?
    Steroids. 2005;70(14):974-9
  16. Herbert Z. ,Gothe S. ,Caldwell JD. ,Bernstein HG. ,Melle C. ,von Eggeling F. ,LEWIS JG. ,Jirikowski GF.
    ",Identification of sex hormone-binding globulin in the human hypothalamus.
    Neuroendocrinology. 2005;81(5):287-93
  17. Markowitz JS. ,Devane CL. ,LEWIS JG. ,Chavin KD. ,Wang JS. ,Donovan JL.
    ",Effect of Ginkgo biloba Extract on Plasma Steroid Concentrations in Healthy Volunteers: A Pilot Study.
    Pharmacotherapy. 2005;25(10):1337-40
  18. LEWIS JG. ,Bagley CJ. ,Elder PA ,Bachmann AW. ,Torpy DJ.
    ",Plasma free cortisol fraction reflects levels of functioning corticosteroid-binding globulin.
    Clin Chim Acta. 2005;359(1-2):189-94
  19. Donovan JL. ,DeVane CL. ,LEWIS JG. ,Wang JS. ,Ruan Y. ,Chavin KD. ,Markowitz JS.
    ",Effects of St Johns wort (Hypericum perforatum L.) extract on plasma androgen concentrations in healthy men and women: a pilot study.
    Phytother Res. 2005;19(10):901-6
  20. Mishra S. ,Storer MK. ,Sherwin CM. ,LEWIS JG.
    ",A simple binding assay for the direct determination of biotin in urine.
    Clin Chim Acta. 2005;360(1-2):60-6
  21. LEWIS JG. ,Shand BI. ,Elder PA ,Scott RS.
    ",Plasma sex hormone-binding globulin rather than corticosteroid-binding globulin is a marker of insulin resistance in obese adult males.
    Diabetes Obes Metab. 2004;6(4):259-63
  22. LEWIS JG. ,Florkowski CM ,Elder PA ,Hunt PJ.
    ",Rheumatoid factor and false positive sex-hormone binding globulin.
    Clin Chim Acta 2003;332(1-2):139-41
  23. Lewis MG. ,LEWIS JG. ,Elder PA ,Moore GA
    ",An enzyme-linked immunosorbent assay (ELISA) for methylphenidate (Ritalin ) in urine.
    J Anal Toxicol. 2003;27(6):342-5.
  24. Lewis MG. ,LEWIS JG. ,Elder PA
    ",An enzyme-linked immunosorbent assay for corticosteroid-binding globulin using monoclonal and polyclonal antibodies: decline in CBG following synthetic ACTH.
    Clin Chim Acta. 2003;328(1-2):121-8
  25. Elder PA ,Hellemans J. ,Dawson T. ,LEWIS JG.
    "The effect of a novel deer velvet extract on human steroid metabolism."
    NZ J Sports Med. 2003;30(3):68-72
  26. Elder PA ,Hellemans J. ,LEWIS JG. ,Dawson T.
    "Tribulus Terrestris ingestion: does it work?"
    NZ J Sports med. 2002;29(4):74-77
  27. Shand BI. ,LEWIS JG. ,Elder PA ,Agarwal M.
    ",Antihypertensive treatment and glomerular size selectivity in hypertensive patients with renal parenchymal disease and proteinuria
    Clin Nephrol. 2002;58(4):321-4
  28. Florkowski CM ,Elder PA ,LEWIS JG. ,Hunt PJ p. ,Munns PL. ,Hunter W. ,Baldwin D.
    ",Two cases of adrenal suppression following a Chinese herbal remedy: a cause for concern?
    N Z Med J. 2002;115(1153):223-4
  29. LEWIS JG. ,Morris JC. ,Clark BM. ,Elder PA
    ",The effect of isoflavone extract ingestion, as Trinovin, on plasma steroids in normal men
    Steroids. 2002;67(1):25-9
  30. LEWIS JG. ,McGill H. ,Patton vm. ,Elder PA
    ",Caution on the use of saliva measurements to monitor absorption of progesterone from transdermal creams in postmenopausal women.
    Maturitas. 2002;41(1):1-6
  31. LEWIS JG. ,Elder PA
    ",Abbreviated direct and indirect ELISAs: a new and simple format.
    Clin Chem. 2000;46(1):137-9
  32. LEWIS JG. ,Longley NJ. ,Elder PA
    ",Monoclonal antibodies to human sex hormone-binding globulin (SHBG): characterization and use in a simple enzyme-linked immunosorbent assay (ELISA) of SHBG in plasma.
    Steroids. 1999;64(4)_:259-65
  33. LEWIS JG. ,Elder PA
    ",Alternative ELISA for sex hormone-binding globulin in plasma.
    Clin Chem. 1998;44(9):1999
  34. Lettink M. ,LEWIS JG. ,Elder PA
    "A monoclonal Antibody raised against amino acids 497-507 of the human estrogen receptor recognises human ataxin-2"
    J.Biochem.Mol.Biol & Biophys. 1998;2:111-119
  35. LEWIS JG. ,George PM ,Elder PA
    ",Plasma androsterone/epiandrosterone sulphates as markers of 5 alpha-reductase activity. Effect of finasteride in normal men
    Steroids 1997;62(8-9):632-635
  36. Domigan NM. ,Carr AC. ,LEWIS JG. ,Elder PA ,Winterbourn CC.
    "A monoclonal antibody recognizing the chlorohydrin derivatives of oleic acid for probing hypochlorous acid involvement in tissue injury."
    Redox Report. 1997;3:111-117
  37. LEWIS JG. ,BasonLM. ,Elder PA
    "Production and characterization of monoclonal antibodies to dehydroepi-androsterone sulfate: Application to direct enzyme-linked immunosorbent assays of dehydroepiandrosterone sulfate and androsterone"
    Steroids 1996;:
  38. Florkowski CM ,Wittert GA. ,LEWIS JG. ,Donald RA. ,Espiner EA.
    ",Glucocorticoid responsive ACTH secreting bronchial carcinoid tumours contain high concentrations of glucocorticoid receptors.
    Clin Endocrinol (Oxf). 1994;40(2):269-74
  39. LEWIS JG. ,Manley L. ,Whitlow JC. ,Elder PA
    ",Re-examining steroid hormone metabolites as ovulation markers using monoclonal antibodies.
    Steroids. 1994;59(4):288-91
  40. Lewis LK. ,Yandle TG. ,LEWIS JG. ,Richards AM. ,Pidgeon GB. ,Kaaja RJ. ,Nicholls MG.
    ",Ouabain is not detectable in human plasma.
    Hypertension. 1994;24(5):549-55
  41. LEWIS JG. ,Elder PA
    "Recycling ovulation markers (letter)."
    NZ Med J 1993;106:165-166
  42. LEWIS JG. ,Manley L. ,Whitlow JC. ,Elder PA
    ",Production of a monoclonal antibody to cortisol: application to a direct enzyme-linked immunosorbent assay of plasma.
    Steroids 1992;57:82-85
  43. Elder PA ,Manley L. ,LEWIS JG.
    ",Use of a monoclonal antibody to estrone-3-glucuronide in an enzyme-linked immunosorbent assay (ELISA).
    J Steroid Biochem 1990;36:439-493
  44. LEWIS JG. ,Clifford JK. ,Elder PA
    ",Monoclonal antibodies to pregnanediol-3-glucuronide: application to a direct enzyme-linked immunosorbent assay of urine.
    Steroids 1990;55:314-318
  45. LEWIS JG. ,Robertson JM. ,Elder PA
    ",A monoclonal antibody to a 48 K antigen in oestrogen-dependent human breast cancer cells.
    J Steroid Biochem 1989;33:171-178
  46. Elder PA ,Yeo KH. ,LEWIS JG.
    ",An ELISA for 17 alpha hydroxyprogesterone.
    Steroids 1988;52:427-428
  47. Yeo KH. ,Elder PA ,LEWIS JG.
    ",An automated solid-phase 17 alpha-hydroxyprogesterone ELISA method using a microtitre plate.
    Ann Clinical Biochem 1988;:293-297
  48. LEWIS JG. ,Elder PA
    ",Affinity chromatography using competitive elution separates polyclonal glucocorticoid antisera into fractions of varying cross-reactivity.
    J Steroid Biochem 1988;29:191-195
  49. Elder PA ,Yeo KH. ,LEWIS JG. ,Clifford JK.
    ",An enzyme-linked immunosorbent assay (ELISA) for plasma progesterone: immobilised antigen approach.
    Clin Chim Acta 1987;162:199-206
  50. LEWIS JG. ,Yeo KH. ,Elder PA
    ",A competitive enzyme-linked immunosorbent assay for plasma 11-deoxycortisol (17,21-dihydroxy-4-pregnene-3,20-dione).
    Steroids 1986;47:365-372
  51. LEWIS JG. ,Elder PA ,Yeo KH.
    ",A monoclonal antibody to prednisone: use of enzyme-linked immunosorbent assay (ELISA) for screening and characterization of antigenic determinants.
    J Steroid Biochem 1986;25:659-693
  52. Robertson JM. ,LEWIS JG. ,Elder PA
    ",Separation methods in the estrogen-receptor assay: dextran-coated charcoal compared with glass fibre.
    Clin Chem 1986;32:2104-2105
  53. LEWIS JG. ,Manley L. ,Townsend JC. ,Elder PA
    ",An enzyme-linked immunosorbent assay (ELISA) for urinary free cortisol.
    Clin Chim Acta 1986;159:205-209
  54. LEWIS JG. ,Elder PA
    ",Fractionation of cortisol antisera by immunoadsorption chromatography: characterisation and use in an enzyme-linked immunosorbent assay (ELISA).
    J Steroid Biochem 1985;22:387-385
  55. LEWIS JG. ,Elder PA
    ",An enzyme-linked immunosorbent assay (ELISA) for plasma cortisol.
    J Steroid Biochem 1985;22:673-676
  56. Elder PA ,LEWIS JG.
    ",An enzyme-linked immunosorbent assay (ELISA) for plasma testosterone.
    J Steroid Biochem 1985;22:635-638
  57. LEWIS JG. ,Andre CM.
    ",The effect of alpha 2HS glycoprotein on lymphocyte reactivity to phytohemagglutinin.
    Immunol Commun. 1984;13(1):35-47
  58. Owen MC. ,Brennan SO ,LEWIS JG. ,Carrell RW.
    ",Mutation of antitrypsin to antithrombin. alpha 1-antitrypsin Pittsburgh (358 Met leads to Arg), a fatal bleeding disorder.
    N Engl J Med. 1983;309(12):694-8
  59. LEWIS JG. ,Andre CM.
    ",Evidence that the alpha 2HS glycoprotein receptor and the Epstein-Barr virus nuclear antigen (EBNA) are identical.
    FEBS Lett. 1983;161(2)1306-10:
  60. LEWIS JG. ,Andre CM.
    ",The alpha 2 HS glycoprotein receptor on lymphocytes transformed by Epstein--Barr virus.
    FEBS Lett. 1982;143(2):332-6
  61. LEWIS JG. ,Crosier PS. ,Andre CM.
    ",alpha 2 HS glycoprotein binds to lymphocytes transformed by Epstein-Barr virus.
    FEBS Lett. 1982;138(1):37-9
  62. LEWIS JG. ,Andre CM.
    ",Binding of alpha 2HS glycoprotein in peripheral blood monocytes.
    Immunol Commun. 1981;10(6):541-7
  63. LEWIS JG. ,Andre CM.
    ",Enhancement of human monocyte phagocytic function by alpha 2HS glycoprotein.
    Immunology. 1981;42(3):481-7
  64. LEWIS JG. ,Andre CM.
    ",Effect of human alpha 2HS glycoprotein on mouse macrophage function
    Immunology. 1980;39(3):317-22
  65. LEWIS JG. ,Andre CM.
    ",A serum DNA-binding protein absent in malignant disease.
    FEBS Lett. 1978;92(2):211-3
  66. Jacaman FR. ,Ansell ID. ,Ghanadian R. ,McLoughlin PV. ,LEWIS JG. ,Chisholm GD.
    ",The hormone response to a synthetic androgen (mesterolone) in oligospermia.
    Clin Endocrinol (Oxf). 1977;6(5):339-45
  67. Ghanadian R. ,LEWIS JG. ,Chisholm GD. ,ODonoghue EP.
    ",Serum dihydrotestosterone in patients with benign prostatic hypertrophy.
    Br J Urol. 1977;49(6):541-4
  68. Ghanadian R. ,LEWIS JG. ,Chisholm GD.
    ",Androgen concentrations in prostate and serum of the male and female Praomys (Mastomys) natalensis.
    Invest Urol. 1977;15(3):212-4
  69. Elder PA ,Gaynor PT. ,LEWIS JG. ,Bodger PS. ,Bason LM.
    "Generation of monoclonal progesterone antibodies by electrofusion techniques."
    Bioelectrochem. Bioenerg. 1977;43:35-40
  70. LEWIS JG. ,Ghanadian R. ,Chisholm GD.
    ",Proceedings: Serum androgens in male and female Praomys (Mastomys) natelensis.
    J Endocrinol. 1976;68(3):27P
  71. LEWIS JG. ,Ghanadian R. ,Chisholm GD.
    ",Serum 5alpha-dihydrotestosterone and testosterone changes with age in man.
    Acta Endocrinol (Copenh). 1976;82(2):444-8
  72. Ghanadian R. ,LEWIS JG. ,Chisholm GD.
    ",Serum testosterone and dihydrotestosterone changes with age in rat.
    Steroids. 1975;25(6):753-62
  73. LEWIS JG. ,Ghanadian R. ,Chisholm GD.
    ",Proceedings: Age-related changes in serum 5alpha-dihydrotestosterone and testosterone in normal men.
    J Endocrinol. 1975;67(2):15P
    Information last updated on 26 August 2009
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